Microgravity might affect Peri - implant Mucosal Epithelial cells during space Flight
نویسنده
چکیده
Materials and methods: Gingival epithelial cells were seeded and cultured at 10−3 G in a three-dimensional clinostat to simulate microgravity (group CL) or in normal gravity (group C) for 10 days. Cell proliferation was analyzed by counting the numbers of cells. Real-time polymerase chain reaction was performed to amplify the krt 5, krt 13 and involucrin genes. Additionally, total protein was immunoblotted with anti-krt 13 antibody. Statistical analysis (n = 9, three groups repeated three times) was performed (ANOVA, Tukey’s test, p < 0.05).
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